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. 2022 Jan 10;8:763141. doi: 10.3389/fmed.2021.763141

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Example of amnion epithelial cell–derived ECV characterization. (A) Representative transmission electron microscopy image. hAEC-ECVs reveal cup-shaped morphology. Scale bar: 50 nm. (B) Size distribution of human amniotic epithelial cell-extracellular vesicles (hAEC-ECVs) obtained by Nanoparticle tracking analysis. The number of particles of hAEC-derived and human dermal fibroblast (HDF)-derived exosomes were plotted as mean ± SD (circle, blue) and mean ± SD with droplines (square, red), respectively. (C) Variation in methodologies of hAEC-ECV isolation and characterization.