Table 1.
Proteomic analysis of human amniotic epithelial cells-extracellular vesicles (hAEC-ECVs).
| (A) Unique proteins in ECV vs. EVDM | GO category | (B) Top represented KEGG pathways | Number of genes | (C) Significantly overrepresented Reactome pathways |
|---|---|---|---|---|
| Programmed cell death protein 6 (Apoptosis-linked gene 2 protein homolog) (ALG-2) | Sorting/Trafficking | hsa04151:PI3K-Akt signaling pathway | 19 | Cellular response to heat stress |
| Histone H2A | Cell adhesion | |||
| CD81 tetraspanin | Tetraspanin | hsa04510:Focal adhesion | 16 | HSF1-dependent transactivation |
| Complement decay-accelerating factor (fragment) | Complement factor | |||
| Integrin beta | Integrin | hsa04015:Rap1 signaling pathway | 11 | Attenuation phase |
| CD59 glycoprotein | Complement factor, GPI anchor | |||
| Prostate stem cell antigen | GPI anchor | hsa04512:ECM-receptor interaction | 8 | Regulation of HSF1-mediated heat shock response |
| Protein S100-A6 (Calcyclin) (Growth factor-inducible protein 2A9) (MLN 4) (Prolactin receptor-associated protein) (PRA) (S100 calcium-binding protein A6) | S100 proteins | |||
| Integrin beta-4 (GP150) (CD antigen CD104) | Wound response, integrin | hsa04612:Antigen processing and presentation | 7 | HSF1 activation |
| Ras-related C3 botulinum toxin substrate 1 (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1) | Wound response |
(A) Examples of proteins unique to hAEC-ECVs in comparison to ECV-depleted medium (EVDM), (B) top 5 represented KEGG pathways of hAEC-ECV proteins, (C) significantly overrepresented Reactome pathways in hAEC-ECVs as compared to human lung fibroblast ECVs. Source: (A,B) (17); supporting information, and (C) (16); supporting information.