FIGURE 1.

Suppression of RCC cells proliferation by NVP-BEZ235, PP242, and Rapamycin in vitro. (A) 1.5 × 10³ or 3.0 × 10³ RCC cells (UMRC6, 786-0, and UOK121) and HEK-293 cells were treated with increasing concentrations of NVP-BEZ235, PP242, and Rapamycin for 48 h. Cell viability was measured with MTT. DMSO-treated cells served as control (0 nM). All value are means ± SD of three replicates. *p < 0.05, versus the corresponding control (0 nM) group; #p < 0.05, versus the corresponding PP242 group; ∆p < 0.05, versus the corresponding Rapamycin group. (B) For colony formation assay, 500 cells of 786-0 and UOK121 were cultured in six-well plate, and cultured with 200 nM of NVP-BEZ235, PP242, and Rapamycin. After 10-14 days of incubation, colonies were stained with Giemsa stain, and counted. Results are representative of three independent experiments.