FIGURE 4.

Dose- and time-dependent regulation of PI3K/Akt/mTOR pathway by NVP-BEZ235, PP242, and Rapamycin. RCC cells (UMRC6, 786-0, and UOK121) were exposed to increasing concentrations (10, 100, 500, and 1,000 nM) of NVP-BEZ235, PP242, and Rapamycin for 48 h, or 200 nM of these compounds for different time periods as indicated, after which the alteration of phosphorylation of (A) Akt, (B) mTOR, and (C) SPOP expression was examined by Western blot respectively. The relative amounts of p-Akt normalized to total Akt, p-mTOR, and SPOP normalized to β-actin, are shown. Cells harvested at 0 h or DMSO-treated cells (0 nM) served as control. Results are representative of three independent experiments.