FIGURE 4.

Müller cells were pre-incubated with TIMP-3 (100 ng/ml) or dilution medium for 1 h before increasing the sugar content of the cultures [25 mM of mannitol as control or 25 mM of glucose (HG)]. After 24 h levels of the p65 subunit of NF-κB [panel (A)], phospho-ERK1/2 [panel (B)], caspase-3 [panel (C)], and ADAM17 [panel (D)] in cell lysates was determined by Western blot analysis. Results are expressed as median (interquartile range) from three different experiments performed in triplicate. Kruskal-Wallis test and Mann-Whitney test were used for comparisons between three groups and two groups, respectively. *p < 0.05 compared with values obtained from cells treated with mannitol. ^#p < 0.05 compared with values obtained from cells treated with HG.