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. 2022 Jan 18;3(1):100499. doi: 10.1016/j.xcrm.2021.100499

Figure 4.

Figure 4

BoDV-1 IgM-ELISA

(A) Cutoffs for each ELISA system are indicated by horizontal lines (green: anti-BoDV-1-N ELISA; red: anti-BoDV-1-X ELISA; blue: anti-BoDV-1-P ELISA). A vertical yellow line indicates the time of death. Cutoffs were determined by 24 characterized negative samples as the sum of arithmetic mean (black horizontal line) plus 6 standard deviations (error line) Green, red and blue horizontal lines represent cutoffs.

(B and C) ELISA results for the current individual and another 7 recently published confirmed BoDV-1-infected individuals. All experiments were carried out once. Interassay variabilities were calculated for N, X, and P antigens in three runs of one sample of individual 2, resulting in 42%, 15%, and 15%, respectively.