REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Cy3 AffiniPure Goat Anti-Human IgG (H+L)	Jackson ImmunoResearch	Cat# 109-165-003; RRID: AB_2337718
Cy3 AffiniPure Goat Anti-Rabbit IgG (H+L)	Jackson ImmunoResearch	Cat# 111-165-003; RRID: AB_2338000
Polyclonal Rabbit Anti-Human IgG/HRP antibody	Agilent	Cat# P021402-2; RRID: AB_2893418
Polyclonal Rabbit Anti-Human IgM/HRP antibody	Agilent	Cat# P021502-2; RRID: AB_2893505
Goat Anti-Rabbit IgG (H+L)-HRP Conjugate antibody	Bio-Rad	Cat# 172-1019; RRID: AB_11125143
Bacterial and virus strains
BoDV-1, 2 strains isolated from human CNS tissue	Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Germany	N/A
Biological samples
Plasma samples of 2 BoDV-1 infected rabbits. The animal experiments were evaluated and approved by the ethics committee of the State Office of Agriculture, Food safety, and Fishery in Mecklenburg – Western Pomerania (LALLF M-V: LVL MV/TSD/ 7221.3-2-010/18).	Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald – Insel Riems, Germany	N/A
Plasma samples of VSBV-1 infected rabbits. The animal experiments were evaluated and approved by the ethics committee of the State Office of Agriculture, Food safety, and Fishery in Mecklenburg – Western Pomerania (LALLF M-V: LVL MV/TSD/ 7221.3-2-010/18).	Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald – Insel Riems, Germany	N/A
Chemicals, peptides, and recombinant proteins
BoDV-1 nucleocapsid Np40 protein, His-tagged	Mikrogen	N/A
BoDV-1 phosphoprotein Pp23, His-tagged	Mikrogen	N/A
BoDV-1 X protein Xp10, GST-tagged	Mikrogen	N/A
PBS	GIBCO	Cat# 14190-094
Tween 20	Caelo	Cat# 3472
Fat free milk powder	Heirler	N/A
RF Absorbent	Virion/Serion	Cat# Z200
Mikrogen TMB	Mikrogen	Cat# 12003
Mikrogen Stopp (24.9% H3PO4)	Mikrogen	Cat# 12004
TRIS buffer with Tween 20, pH 8.0	Sigma-Aldrich	Cat# T9039-10PAK
TaqPath™1-Step RT-qPCR Master Mix, CG	Applied Biosystems	Cat# A15299
NxtScript Reaction Mix	Roche/Penzberg	Cat# 07368372103
NxtScript RT 85U/μL	Roche/Penzberg	Cat# 07371527103
ROX-Reference Dye	Life technologies	Cat# 12223-012
RNase free DEPC water	Roth	Cat# 143.3
PageRuler Prestained Protein Ladder	Thermo Fisher Scientific	Cat# 26617
Roti-Block	Roth	Cat# A151.2
Critical commercial assays
EZ1 Virus Mini Kit v2.0	QIAGEN	Cat# 179799
Deposited data
Dataset uploaded to Mendeley	This study	https://doi.org/10.17632/wpvkdhmgz4.1
Experimental models: Cell lines
Vero cells	Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Germany	N/A
Oligonucleotides
BoDV-1 Mix 1 (target: x/p gene):	Metabion; (Schlottau et al.2)	N/A
• BoDV1-1288-F (TAGTYAGGAGGC TCAATGGCA)
• BoDV-1-1449-R (GTCCYTCAGGA GCTGGTC)
• BoDV-1-1346-FAM (FAM-AAGAAG ATCCCCAGACACTACGACG-BHQ1)
BoDV-1 Mix 6 (target: m/g gene):	Metabion; (Schlottau et al.2)	N/A
• BoDV-1-2262-F (CAATYAATGCAG CYTTCAATGTCTT)
• BoDV-1-2336-R (GAATGTCYGGG CCGAGAG)
• BoDV-1-2316-FAM (FAM-CCARCA CCAATGTTCCGAAGCCG-BHQ1)
MS2:	Metabion;	N/A
• MS2 forward primer	Metabion;
• MS2 reverse primer	Applied Biosystems;
• MS2 So VIC/Tamra	(Dreier et al., 2005)
Software and algorithms
GraphPad Prism V9.2.0	GraphPad	https://www.graphpad.com
Other
Nunc Maxisorp Plates	Thermo Fisher Scientific	Cat# 446469
μ-Plate 96 Well Black	Ibidi	Cat# 89626
Nitrocellulose western blotting membrane Amersham Protran Premium 0.2 μm	Thermo Fisher Scientific	Cat# 15279794