FIGURE 6.

The engineered strain P. putida KTΔAB (p2-acs-phaJ) was used to produce mcl-PHA by fermentation with mixed substrates (A) P. putida KTΔAB (p2-acs-phaJ) was used to produce mcl-PHA in a two-step fermentation with a mixed substrate comprising 10 g/L glucose and 2 g/L octanoate (B) P. putida KTΔAB (p2-acs-phaJ) was cultured with 5 g/L glucose and 5 g/L acetic acid as a substrate for 24 h, after which 2 g/L octanoate was added, and the fermentation was continued for 72 h to measure the final mcl-PHA titer. The error bars indicate the standard deviation of triplicate experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.