Reagent or resource	Source	Identifier
Antibodies
FGF21	Abcam	Cat# ab171941; RRID:AB_2629460
β-ACTIN	Cell Signaling Technology	Cat# 3700S; RRID:AB_2242334
LC3B	Novus Biologicals	Cat# NB100-2220; RRID:AB_10003146
p62/SQSTM1	Abcam	Cat# ab56416; RRID:AB_945626
GHRL	Santa Cruz	Cat# sc-517596; RRID: NA
phospho-mTOR (Ser2448)	Cell Signaling Technology	Cat# 5536S; RRID:AB_10691552
mTOR	Cell Signaling Technology	Cat# 2983S; RRID:AB_2105622
phospho-ULK1 (Ser757)	Cell Signaling Technology	Cat# 14202S; RRID:AB_2665508
ULK1	Cell Signaling Technology	Cat# 8054S; RRID:AB_11178668
phospho-p70 S6 Kinase (Thr389)	Cell Signaling Technology	Cat# 9234S; RRID:AB_2269803
p70 S6 Kinase	Cell Signaling Technology	Cat# 2708S; RRID:AB_390722
phospho-4E-BP1 (Thr37/46)	Cell Signaling Technology	Cat# 2855S; RRID:AB_560835
4E-BP1	Cell Signaling Technology	Cat# 9644S; RRID:AB_2097841
Horse Anti-Mouse IgG-HRP	Cell Signaling Technology	Cat# 7076S; RRID:AB_330924
Goat Anti-Rabbit IgG-HRP	Cell Signaling Technology	Cat# 7074S; RRID:AB_2099233
Bacterial and virus strains
AAV8-eGFP	Vector Biolabs	Cat# 1060
AAV8-TGB-arcA	Vector Biolabs	NA
Ad-CMV-eGFP	Vector Biolabs	NA
Ad-CMV-arcA-eGFP	Vector Biolabs	NA
Biological samples
Fetal Bovine Serum (FBS)	GIBCO	Cat# 26140-079
Chemicals, peptides, and recombinant proteins
ADI-PEG 20	Polaris Pharmaceuticals Inc.	Kit# 36386
TRIzol	Invitrogen	Cat# 15596018
0.9% Sodium Chloride Injection, USP	Hospira
Fast SYBR Green Master Mix	Applied Biosystems	Cat# 4385612
Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA-Free	Thermo Scientific	Cat# A32961
10X TBST	EZ BioResearch	Cat# S-1012
10X Tris/Glycine Buffer	Bio-Rad	Cat# 1610734
10X Tris/Glycine/SDS Buffer	Bio-Rad	Cat# 1610732
Mini-PROTEAN TGX Stain-Free Gels	Bio-Rad	Cat# 4568094
Clarity Western ECL Substrate	Bio-Rad	Cat# 1705060
Humulin R	Lilly USA, LLC	NDC 0002-8215-17
Ultra Sensitive Mouse Insulin ELISA Kit	Crystal Chem	Cat# 90080
Mouse/Rat Fibroblast Growth Factor 21 ELISA	BioVendor	Cat# RD291108200R
Glucose Colorimetric Assay Kit	Cayman Chemical	Cat# 10009582
Triglycerides Standard	Pointe Scientific	Cat# T7531-STD
Infinity Triglycerides	Thermo Scientific	Cat# TR22421
Cholesterol Standard	Stanbio	Cat# 1012-030
Infinity Cholesterol	Thermo Scientific	Cat# TR13421
NEFA Standard Solution	Fujifilm	Cat# 276-76491
HR Series NEFA-HR(2) Color Reagent A	Fujifilm	Cat# 999-34691
HR Series NEFA-HR(2) Solvent A	Fujifilm	Cat# 995-34791
HR Series NEFA-HR(2) Color Reagent B	Fujifilm	Cat# 991-34891
HR Series NEFA-HR(2) Solvent B	Fujifilm	Cat# 993-35191
HDL-C/LDL-C Calibrator	Fujifilm	Cat# 990-28011
L-Type LDL-C Reagent 1	Fujifilm	Cat# 993-00404
L-Type LDL-C Reagent 2	Fujifilm	Cat# 999-00504
Albumin from Bovine Serum	Sigma-Aldrich	SKU A3983-50G
2-Propanol	Sigma-Aldrich	SKU 190764-500ML
Methanol	Sigma-Aldrich	SKU 179337-4L-PB
Chloroform	Sigma-Aldrich	SKU 319988-500ML
Ethanol	Decon Laboratories, Inc.	Cat# 2701
D-(+)-Glucose	Sigma-Aldrich	SKU G8270-100G
DMEM/F12(1:1) (1X)	GIBCO	Cat# 11330-032
Insulin-Transferrin-Selenium 100X	GIBCO	Cat# 41400-045
Dexamethasone	Sigma-Aldrich	SKU D4902-100MG
Pen Strep	GIBCO	Cat# 15140-122
Rodent Diet: Adjusted Calories Diet (42% from fat)	Envigo Teklad Diets	Cat# TD.88137
Nuclease-Free Water	Invitrogen	Cat# AM9937
Critical commercial assays
Seahorse XF Cell Mito Stress Test Kit	Agilent	Cat# 103015-100
QuantiTect Reverse Transcription Kit	QIAGEN	Cat# 205314
Deposited data
Gene Expression RNA-seq data	This paper	GEO: GSE191295
Single-cell ATAC-seq data	This paper	GEO: GSE192413
Experimental models: Cell lines
Mouse: AML12	ATCC	Cat# CRL-2254; RRID:CVCL_0140
Experimental models: Organisms/strains
Mouse: C57B/J6	Jackson Laboratory	RRID: IMSR_JAX:000664
Mouse: db/db	Jackson Laboratory	RRID: IMSR_JAX:000642
Mouse: Becn1+/−	Jackson Laboratory	RRID: IMSR_JAX:018429
Mouse: Becn1 flox	Jackson Laboratory	RRID: IMSR_JAX:028794
Mouse: Alb1-cre	Jackson Laboratory	RRID: IMSR_JAX:016832
Mouse: Fgf21 flox	Jackson Laboratory	RRID: IMSR_JAX:022361
Oligonucleotides
qPCR Primers	This paper	Table S1
Software and algorithms
GraphPad Prism 7	GraphPad Software Inc.	http://www.graphpad.com; RRID:SCR_002798
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/; RRID:SCR_003070