Figure 4.

The base and 3₁₀ pocket domains. The base domain lies below the glycan cap and above the very bottom of GP, and contains the cryptic 3₁₀ pocket (green), which is occupied by the bottom of the β17-β18 loop in unliganded/uncleaved GP. Broadly neutralizing antibodies gain access to the 3₁₀ pocket by mimicking and displacing the β17-β18 loop, typically through extended CDRH3 loops. ADI-15946 (orange, PDBID: 6MAM) has a smaller 3₁₀ footprint and thus less broad neutralizing activity. EBOV-520 (purple, PDBID: 6PCI) also binds to regions outside of the 3₁₀ pocket and thus slightly lower activity toward more divergent ebolavirus species like SUDV. EBOV-515 (pink, PDBID: 7M8L) has the broadest neutralizing activity due to a targeted footprint toward the most conserved portions of the 3₁₀ pocket.