TABLE 1.
Overview of the experimental conditions.
| Experiment no. and general conditions | Cultivation name/process mode | Addition of CaCO3 a | Feed/exchange medium |
|---|---|---|---|
| No. 1 Feeding and exchange rate b : every 3 days; temperature: 35°C | Batch A | — | — |
| FB A | — | Glucose | |
| FB B | — | Glucose and N-source | |
| FB C | — | Complete medium c | |
| RB A | 6 g after every exchange | Complete medium c | |
| RB B | 3 g after every exchange | Complete medium c | |
| No. 2 Feeding and exchange rate b : every 2 days; temperature: 32°C | Batch B | — | — |
| RB C | Regular additions of 2.5 g as needed d | Complete medium c | |
| RB D | t = 336 h: 9 g and t > 336 h: Regular additions of 2.5 g as needed d | Complete medium c | |
| RB E | Regular additions of 2.5 g as needed d | Complete medium c and without N-source |
a
Starting concentration of calcium carbonate of all cultivations was 90 g/L.
b
Feeding and exchange rates after the initial growth phase of 4 days.
c
The “complete medium” comprises all components of the batch medium as listed in the Materials and Methods section, except for sodium chloride.
d
“As needed” means that calcium carbonate was only added in case the medium has cleared up.