Figure 1.

Schematic representation of the tissue processing workflow for CRC organoid generation. Upon collection, CRC tissues were divided into 5 similar pieces. Each sample underwent a different washing condition as described in the methods (left). Samples were washed three times for 5 min while maintained on ice. After the washing step, all the tissues were processed using the same protocol and conditions. A cell suspension was generated using mechanical and enzymatic digestion that was then embedded in Matrigel and daily monitored for presence of microbial contamination. Created with BioRender.com.