Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Illustrated flow chart of PDO generation. (A) Tissue cutting. (B) Washing steps. (C) Tissue mincing. (D) Enzymatic tissue dissociation. (E) Generation of single cell suspension. (F) Cell count and viability assessment. (G) Cell embedding in Matrigel. (H) Addition of supplemented PDO-CRC medium.
