Table 3B.

Flow cytometry results^* stratified for urine recirculation and urine replacement.

	Kidneys with urine recirculation (n = 8)			Kidneys without urine recirculation (n = 4)			p-value***30 min	p-value*** 1 h	p-value*** 6 h
	30 min**	1 h**	6 h**	30 min**	1 h**	6 h**
Total leukocytes	194,687, 169,462	301,435, 173,632	285,198, 314,757	112,250, 125,008	173,140, 224,651	131,250, 371,186	0.37	0.21	0.68
Total T Cells	39,778, 42,889	54,203, 18,377	79,875, 79,263	21,809, 28,131	26,523, 47,966	28,027, 69,184	0.28	0.15	0.28
CD4 T cells	14,579, 24,059	24,088, 14,444	30,468, 54,969	6,123, 9,731	8,402, 11,934	9,112, 40,426	0.28	0.07	0.57
CD8 T cells	16,913, 13,757	33,008, 19,555	31,977, 21,867	11,074, 18,395	13,174, 32,824	12,905, 23,162	0.15	0.15	0.11
NK T cells	2,943, 5,681	4,595, 3,800	8,050, 11,446	6,254 ± 3,241	7,870, 5,795	7,932, 5,671	0.68	0.37	0.93
B cells	17,692, 29,619	18,707, 26,760	31,089, 43,484	18,933, 64,538	16,183, 60,774	19,205, 271,378	0.68	0.99	0.93
Monocytes
Classical	6,310, 10,731	18,017, 19,859	7,188, 11,711	7,480, 2,978	15,596, 19,757	5,547, 5,020	0.99	0.93	0.99
Intermediate	3,462, 5,310	9,255, 11,880	1,363, 2,799	4,180, 3,842	13,508, 13,273	1,292, 1,749	0.81	0.46	0.93
Non-classical	2,743, 3,285	7,667, 6,711	3,790, 3,645	1,810, 3,074	2,960, 2,963	1,114, 1,364	0.15	0.05	0.05
Eosinophils	545, 12,346	613.4, 5,528	1,122, 24,421	332, 764	296, 853	711, 653	0.57	0.28	0.81
Neutrophils	157, 202	379.1, 156.2	210.2, 351.4	105, 392	296, 438	1,039, 2,564	0.88	0.49	0.20
NK cells	11,780, 56,253	34,918, 63,636	26,187, 48,707	10,316, 12,633	19,607, 22,801	3,549, 5,731	0.57	0.21	0.07
Macrophages	3,073, 9,088	4,433, 11,636	3,709, 4,719	6,469, 6,217	21,035, 42,213	5,988, 15,180	0.68	0.21	0.46

^*Number of cells in cells/ml; overall perfusate volume = 500 ml.

^**Time after start of NMP, values in median and IQR (interquartile range).

^***P-value result of comparison with and without urine recirculation.