Table 8.

Weight of evidence and uncertainty analysis of the available laboratory data for aquatic organisms. EV = external validity; IV = internal validity; Prec = precision; IC = internal consistency

Assessment endpoint group	RefID|exp	Strength of the line of evidence	Potential to indicate a higher hazard compared to EFSA (2016)
			Judgement	Rationale
Survival	acute 28|1	EV: low RoB IV: low RoB Prec: low IC: NA	High with a high certainty for fish Low with a high certainty for aquatic organisms as a whole	The data are relevant for the risk assessment of aquatic organisms, but as the aquatic risk assessment is driven by aquatic invertebrates the overall potential of the new study with fish to indicate a higher hazard is low. Overall, the data seem robust. Since only one study with this species is available, the internal consistency of the line of evidence cannot be assessed
	chronic 16|1 16|3	EV: moderate RoB IV: high RoB Prec: low IC: NA	Low with a low certainty	The data are relevant for the risk assessment of aquatic organisms, but as the previous endpoint for aquatic invertebrates is lower than the tested concentrations the potential of the new study with snails to indicate a higher hazard is low. Overall, the data seem not robust as the concentrations were not measured. Since only one study with this species is available, the internal consistency of the line of evidence cannot be assessed
Reproduction	chronic 16|4	EV: moderate RoB IV: high RoB Prec: low IC: NA	Low with a low certainty	The data are relevant for the risk assessment of aquatic organisms, but as the previous endpoint for aquatic invertebrates is lower than the tested concentrations the potential of the new study with snails to indicate a higher hazard is low. Overall, the data seem not robust as the concentrations were not measured. Since only one study with this species is available, the internal consistency of the line of evidence cannot be assessed
Subindividual	Acute and chronic 28|2 28|3 16|1 16|3	EV: high RoB IV: low to high RoB Prec: low IC: NA	Low with a moderate certainty	The data are of limited relevance for the risk assessment, due to the impossibility to link subindividual effects to effects at the population level. The potential to indicate a higher hazard is low as the tested concentrations were higher than the previous endpoint triggering the aquatic risk assessment. Overall, the data seem not robust as the concentrations were not measured. Since only one study with this species is available, the internal consistency of the line of evidence cannot be assessed