Table A.1 Outline of the appraisal questions for aquatic organisms

Section	Acronym	Question
External validity	Q1_EV	How confident are we that the assessment endpoint can be used to inform the risk assessment of aquatic organisms?
	Q2_EV	Are the test organisms exposed to either flupyradifurone or acetamiprid in isolation (without any other active substances)?
	Q3_EV	Are the tested organisms relevant for Europe?
	Q4_EV	Is the duration of the exposure and observation in line with the standard testing?
Internal validity	Q1_IV	Is the origin of the tested organism trustable?
	Q2_IV	Is the age and sex of the tested organisms known and appropriate?
	Q3_IV	Were the test organisms properly acclimatised to the study setup before the exposure started?
	Q4_IV	Are the test organisms healthy and stress‐free at the start of the experiment?
	Q5_IV	Is the methodology used (including the experimental setup) for measuring the assessment endpoint reliable?
	Q6_IV	Is the negative (blank) control performing adequately?
	Q7_IV	If a solvent is used, is the effect of the solvent appropriately accounted for?
	Q8_IV	Are the test conditions appropriate? This includes relevant OECD validity criteria concerning optimal tests conditions for the tested species (e.g. oxygen saturation).
	Q9_IV	Is the test item clearly identified and characterised?
	Q10_IV	Is exposure characterised by analytical measurements and is it maintained during the test duration?
	Q11_IV	Was a clear dose response observed in the study?
	Q12_IV	Is the derivation of the measured endpoint(s) performed with sound statistical methods?
Precision	Q1_PR	Are the sample size and replication appropriate?
	Q2_PR	Is the number of tested concentrations/doses appropriate?
	Q3_PR	Is doses selection (including the space between them) appropriate?