Table 2.
Measured parameters of sucrose test reported in CMS studies
| Rat strain | Stress period (weeks) | Sucrose concentration (%) | Adaptation to sucrose (days) | Food and water deprivation prior test (hrs) | Test duration (hrs) | Mean of sucrose intake (g) | Mean of sucrose preference (%) | Author | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Stress group | No stress control | Stress group | Baseline |
No stress control |
|||||||
| Wistar | 9 | 1.5 | 35 | 14 | N.A. | 5 | 14 | N.A. | N.A. | N.A. | Czéh et al. 2018 |
| Wistar | 5 | 1 | 2 | 24 | 3 | N.A. | N.A. | 50 | N.A. | 90 | Fan et al. 2018 |
| Sprague- Dawley | 4 | 1 | 2 | 4 | 1 | N.A. | N.A. | 55 | N.A. | 80 | Li et al. 2018 |
| Sprague- Dawley | 3 | 1 | 4 | 24 | 1 | N.A. | N.A. | 40 | N.A. | 82 | Lu et al. 2018 |
| Sprague- Dawley | 3 | 1 | N.A. | 12 | 12 | N.A. | N.A. | 70 | N.A. | 100 | Song et al. 2018 |
| Sprague- Dawley | 3 | 1 | N.A. | N.A. | 24 | N.A. | N.A. | 55 | 80 | 80 | Zhang et al. 2018 |
| Sprague- Dawley | 3/7/28 days | 1 | 2 | 4 | 1 | N.A. | N.A. | 66 | N.A. | 84 | Franklin et al. 2018 |
| Sprague-Dawley | 5 | 2 | N.A. | N.A. | 3 | N.A. | N.A. | 67 | N.A. | 76 | Kreisel et al. 2014 |
| Sprague-Dawley | 3 | 1 | 2 | 4 | 1 | N.A. | N.A. | 50 | N.A. | 65 | Li et al. 2011 |
| Sprague-Dawley | 35 days | 1 | 2 | 4 | 1 | N.A. | N.A. | 69 | N.A. | 75 | Banasr et al. 2010 |
| Wistar | 6 | 1 | 7 | 18 | 1 | N.A. | N.A. | 75 | 90 | 90 | Bessa et al. 2009 |
| Sprague-Dawley | 35 days | 1 | 2 | 4 | 1 | N.A. | N.A. | 33 | N.A. | 75 | Banasr and Duman 2008 |
| Sprague-Dawley | 3 | 1 | 2 | 4 | 1 | N.A. | N.A. | 35 | N.A. | 70 | Koo and Duman. 2008 |
| Sprague-Dawley | 8, 15, or 35 days | 1 | 2 | 4 | 1 | N.A. | N.A. | 33 | N.A. | 75 | Banasr et al. 2007 |
| Rat strain | Stress period (weeks) | Sucrose concentration (%) | Adaptation to sucrose (days) | Food and water deprivation prior test (hrs) | Test duration (hrs) | Mean of sucrose intake (g) | Mean of sucrose preference (%) | Author | |||
| Stress | No stress group control | Stress group | Baseline |
No stress control |
|||||||
| Wistar | 10 | 1 | N.A. | 15 | 1 | 7 | 12 | N.A. | N.A. | N.A. | Bortolato et al. 2007 |
| Sprague-Dawley; Wistar | 24 days | 1.5 | 35 | 18 | 1 | 7 | 15 | N.A. | N.A. | N.A. | Lucas et al. 2007 |
| Sprague-Dawley | 2 | 1 | N.A. | 2.5 | 1 | N.A. | N.A. | 60 | N.A. | 80 | Lu et al. 2006 |
| Wistar | 4 | 1.5 | 35 | 18 | 1 | 7.5 | 16 | N.A. | N.A. | N.A. | Jayatissa et al. 2006 |
| Wistar | 6 | 1 | 28 | 14 | 1 | 5 | 10 | N.A. | N.A. | N.A. | Dalla et al. 2005 |
| Wistar | 7 | 1 | 7 | 23 | 1 | 2 | 5 | 45 | 66 | 66 | Bekris et al. 2005 |
| Sprague-Dawley | 7 | 1 | 7 | 23 | 1 | 2.8 | 3.2 | 40 | 78 | 75 | |
| Wistar | 9 | 1 | 10 1-hr sessions | 14 | 1 | 7.3 | 14 | N.A. | N.A. | N.A. | Papp et al. 2003 |
| Lister Hooded | 9 | 1 | 2 | 19 | 1 | 6 | 10 | N.A. | N.A. | N.A. | Daquila et al. 1994 |
| Wistar | 7 or 8 | 1 | 5 1-hr sessions | 14 | 1 | 7 | 13.5 | N.A. | N.A. | N.A. | Papp and Moryl, 1994 |
| Lister Hooded | 4 | 0.7 | N.A. | 20 | 1 | 6 | 8 | N.A. | N.A. | N.A. | Papp et al. 1991 |
Reviewed twenty-five studies utilizing CMS procedures on male rats employed various modifications of 1-h sucrose consumption test, where concentrations of sucrose solution, duration of water deprivation period and measured parameters were variables. The results demonstrated a broad range of changes across compared studies in stressed and non-stressed groups
Modifications of 1-h sucrose preference test protocols were reported in twenty-five studies utilizing CMS procedures on male rats. Observed variety of experimental modifications regarding concentration of sucrose solution, duration of a deprivation period and other parameters might result in broad range of values in stress vs. non-stressed groups across studies. Abbreviation: N.A. - Information is not available. Recent studies (published since 2018 and with greater than or equal to 25 citations) are indicated by bold font