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. Author manuscript; available in PMC: 2022 Jan 24.
Published in final edited form as: Adv Healthc Mater. 2021 Mar 18;10(9):e2002030. doi: 10.1002/adhm.202002030

Figure 6.

Figure 6.

Relative mRNA expression of articular cartilage specific genes. Gene expression was investigated three days (Day 3) after encapsulation under free swelling conditions (a) and after one week (Day 10) of dynamic compression (b). Dynamic loading data is normalized by free swelling controls at the same time point (Day 10). Data represent expression of genes encoding for collagen X (COL10A1), collagen I (COL1A1), matrix metalloproteinase 13 (MMP13), SRY-box transcription factor 9 (SOX9), and collagen II (COL2A1). Data from four hydrogels were averaged for each condition, excluding samples which did not provide reliable amounts of mRNA (n=3–4) showing mean and standard error. Data were normalized to ribosomal protein L30 (L30) using the ΔΔCq method.[65] Statistical significance represents the result of a two-way ANOVA where # = P < 0.05 between free swelling and dynamic compression at Day 10 and a one-way ANOVA with Tukey’s MCT where * = P < 0.05 for differences between hydrazone CAN formulations.