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. 2022 Jan 24;13:26. doi: 10.1186/s13287-022-02705-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — In vitro chondrogenic differentiation analysis of 3D-cultured rASCs. A Quantitative real-time PCR analysis of the expression of chondrogenic differentiation-related genes in rASCs encapsulated in GelMA and GelMA/PAMAM-MA hydrogels. B Representative images of safranin-O staining after 14 days of chondrogenic induction culture. Scale bars: above, 200 μm; below, 40 μm. C Representative images of immunofluorescence staining of SOX9 in rASCs encapsulated in two groups of hydrogels after 21 days of chondrogenic induction culture. Scale bars: 75 μm. D Quantification of the positively stained area of GAGs in the two groups of hydrogels. E Quantification of SOX9 expression in the two groups of hydrogels. Error bar: Mean ± SD, * represents p < 0.05