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. 2022 Jan 24;18:54. doi: 10.1186/s12917-022-03142-6

Table 1.

The sequence of primers of Chlamydiales and C. abortus used in the PCR assay

References Length (bp) Sequence (5′–3′) Genes
Borel et al, 2006 [21] 352 bp

Forward 5′-CAA GGT GAG GCT GAT GAC-3′

Reverse 5′-TCG CCT KTC AAT GCC AAG-3’

Chlamydiales

16 s–23 s spacer region

(100pmoles/ ul)

Longbottom et al 2002 [22] 222 bp

Forward 5’- TTTTCAGGATCCTATTGTCCTCCAGGCA −3′

Reverse 5′- GTGTCGACATCAGCATAAATAGCCCCG−3′

Chlamydia. abortus

pmp1 gene

(100 pmoles/ ul)