Table 2.

Primers and TaqMan probes used for detection of C. abortus and internal amplification control by real-time PCR

Name	Sequence	Amplicon size (bp)
CpaOMP1-F CpaOMP1-R CpaOMP1-Sa	5-GCAACTGACACTAAGTCGGCTACA-3 5-ACAAGCATGTTCAATCGATAAGAGA-3 FAMb- TAAATACCACGAATGGCAAGT TGGTTTAGCG-TAMRAc	82
EGFP-1-F EGFP-10-R EGFP-HEXd	5-GACCACTACCAGCAGAACAC-3 5-CTTGTACAGCTCGTCCATGC-3 HEXd- AGCACCCAGTCCGCCCTGAGCABHQ1 e	177

^a Conventional TaqMan probe

^b FAM 6-carboxy-fluorescein

^c TAMRA 6-carboxy-tetramethylrhodamin

^d HEX hexachlorofluorescein

^e Black hole quencher