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. 2022 Jan 5;53(2):381–390. doi: 10.1161/STROKEAHA.121.037537

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© 2022 The Authors.

Stroke is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Figure 4. — Enhanced immunosuppressive activity in female Tregs is associated with intrinsic transcriptional differences. Tregs were sorted via FACS from spleens of depletion of regulatory T cells transgenic (DEREG) mice 24 h after hypoxia-ischemia (HI) to assess their inhibitory capacity on myeloid cell activation measured by quantification of reactive oxygen species production upon stimulation in the absence and presence of Tregs (A). Suppressive activity of Tregs on effector T cells was measured in proliferation assays (B). Serum estradiol levels were analyzed via ELISA 48 h after HI (C). Sorted splenic Tregs were used for microarray analyses, gene enrichment plots including the 10 leading edge genes of the 3 highest enriched gene sets in females compared with males are shown (C). *P<0.05. n=4–5/group in A and B, n=7/group in C, n=3 samples per group (2 animals pooled per sample) in D.