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. 2022 Jan 24;41:33. doi: 10.1186/s13046-021-02230-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Knockdown of RPS27a induces p53-dependent cell cycle arrest and RPL11-dependent p53 activation in A549 cells. A-E A549 cells were co-transfected with p53 and RPS27a-siRNA, and H1299 cells were transfected with RPS27a-siRNA. The expression of proteins was detected with IB (A). The expression of proteins was quantified (target protein/β-actin), and the normalized target protein in NC cells was set at 1.0. *p < 0.05, **p < 0.01 and ***p < 0.001 were calculated with the t-test between co-transfected cells and cells transfected with RPS27a-siRNA alone (n = 3) (B). The cell cycle percentages were analyzed with flow cytometry. **p < 0.01, **p < 0.001 were calculated with ANOVA in A549 cells (n = 3). **p < 0.01 was calculated with the t-test in H1299 cells (n = 3) (C). The percentage of cell apoptosis was analyzed with flow cytometry. ***p < 0.001 was calculated with the t-test between co-transfected cells and cells transfected with RPS27a-siRNA alone (n = 3). **p < 0.01 was calculated with the t-test in H1299 cells (n = 3) (D). The cell viability was measured with CCK-8 assays. ***p < 0.001 was calculated with ANOVA in A549 cells (n = 5). **p < 0.01 was calculated with the t-test in H1299 cells (n = 5) (E). F-J A549 cells were co-transfected with RPL11 and RPS27a-siRNA. The expression of proteins was quantified (target protein/β-actin), and the normalized target protein in NC cells was set at 1.0 (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 were calculated with t-test between co-transfected cells and cells transfected with RPS27a-siRNA alone (n = 3) (F). The cell cycle percentages were analyzed with flow cytometry. ***p < 0.001 were calculated with ANOVA in A549 cells (n = 3) (G). The percentage of cell apoptosis was analyzed with flow cytometry. ***p < 0.001 was calculated with the t-test between co-transfection and transfected with RPS27a-siRNA alone (n = 3) (H). The cell viability was measured with CCK-8 assays. ***p < 0.001 was calculated with ANOVA in A549 cells (n = 5) (I). NC, negative control; IB, immunoblotting