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. 2022 Jan 24;53:7. doi: 10.1186/s13567-022-01025-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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ASFV MGF360-11L inhibited the activation of IFN-β and the ISRE promoter. HEK-293 T cells were co-transfected with IFN-β-Luc (100 ng), ISRE-Luc (100 ng), and pRL-TK (10 ng) plasmids and the IFN-I signaling molecule plasmids cGAS (100 ng, A), STING (100 ng, A), STING (200 ng, B), TBK1 (200 ng, C), IKKε (200 ng, D), IRF3-5D (200 ng, E), and IRF7 (200 ng, F), along with increasing doses of MGF360-11L (50, 100, 200 ng) plasmid or empty vector plasmid (pCMV-N-HA). 24 h post-transfection, cell lysates were used for dual-luciferase reporter assays. The expression of cGAS, STING, TBK1, IKKε, IRF, IRF7 and MGF360-11L was analyzed by Western blotting. All assays were independently repeated at least three times. The data are shown as the mean ± SD; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. Luc: luciferase.