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. 2022 Jan 24;53:7. doi: 10.1186/s13567-022-01025-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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ASFV MGF360-11L could interact with TBK1 and IRF7. The MGF360-11L-HA (1 μg) plasmid was co-transfected with Flag-tagged IFN-I signaling molecule plasmids for cGAS (1 μg), STING (1 μg), TRAF3 (100 ng), TRAF6 (200 ng), TBK1 (1 μg), IKKi (500 ng), IRF3 (500 ng) and IRF7 (1 μg) into HEK-293 T cells, followed by IP with anti-Flag agarose affinity gel. The MGF360-11L-HA plasmid (1 μg) was co-transfected with Flag-tagged IFN-I signaling molecule plasmids for cGAS (1 μg), STING (1 μg), TRAF3 (100 ng), TBK1 (500 ng), IRF3 (200 ng) and IRF7 (1 μg) into HEK-293 T cells, followed by IP with anti-HA agarose affinity gel. Co-IP and Western blotting were carried out using anti-HA-HRP and anti-flag-HRP antibodies. All experiments were independently repeated at least three times. The data are shown as the mean ± SD; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. IB, immunoblotting; HA, anti-HA-tagged monoclonal antibody; IP, immunoprecipitation; WCL, whole cell lysate.