Table 1:
Spinal MN differentiation protocols used in ALS studies
| Reference | Induction compounds | Protocol length (days) |
Culture Purity (%) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| SMAD inhibition | Patterning | ||||||||||
| Chir | LDN | SB | Compound C |
RA | SHH | SAG | HB9 | Tuj1 | ChAT | ||
| (Dimos et al., 2008) | ✓ | ✓ | 45 | 20 | - | - | |||||
| (Hu and Zhang, 2009) | ✓ | ✓ | 56 | >40 | 30-40 | - | |||||
| (Karumbayaram et al., 2009) | ✓ | ✓ | 90-95 | - | - | - | |||||
| (Patani et al., 2011) | ✓ | 55 | - | - | - | ||||||
| (Amoroso et al., 2013) | ✓ | ✓ | ✓ | ✓ | 35 | - | - | - | |||
| (Sareen et al., 2013) | ✓ | 75 | - | - | - | ||||||
| (Maury et al., 2015) | ✓ | ✓ | ✓ | ✓ | ✓ | 35 | - | - | - | ||
| (Qu et al., 2014) | ✓ | ✓ | 30 | - | 70 | - | |||||
| (Kiskinis et al., 2014) | ✓ | ✓ | ✓ | ✓ | 55 | - | - | - | |||
| (Devlin et al., 2015) | ✓ | ✓ | ✓ | 130 | 44 | 81 | - | ||||
| (Du et al., 2015) | ✓ | ✓ | ✓ | 28 | - | - | - | ||||
| (Calder et al., 2015) | ✓ | ✓ | ✓ | 20 | 40 | - | - | ||||
| (Bursch et al., 2019) | ✓ | ✓ | ✓ | ✓ | ✓ | >42 | - | 89 | 27 | ||
| (Dafinca et al., 2020) | ✓ | ✓ | ✓ | ✓ | 30 | - | 90 | 90 | |||
| (Ababneh et al., 2020) | ✓ | ✓ | ✓ | ✓ | 30 | 35 | 80 | 80 | |||
| (Mehta et al., 2021) | ✓ | ✓ | ✓ | ✓ | ✓ | 35 | - | - | - | ||