Table 2:

Cortical neuron differentiation protocols used in C9orf72-ALS/FTD studies

Reference	Induction compounds	Expansion phase	Final maturation factors	Protocol length (days)
(Shi et al., 2012) Used later in (Dafinca et al., 2016)	Dorsomorphin (10 uM) SB (10 uM) N2, B27	Neurospheres	-	80
(Almeida et al., 2013) Used later in (Lopez-Gonzalez et al., 2016; Lopez-Gonzalez et al., 2019; Maor-Nof et al., 2021)	bFGF, N2, B27	Neurospheres	BDNF, GDNF	72
(Bilican et al., 2012) (Livesey et al., 2014) Used later in (Selvaraj et al., 2018)	N2, B27, Forskolin (10 μM)	Monolayer	BDNF, GDNF	56