Fig. 1.

The Rgs21 promoter drives gene expression levels in lingual epithelium that are not equivalently observed in airway or intestinal epithelia. (A–E) Indicated tissue sections from Rgs21::TagRFP transgenic mice or wild-type (WT) mice were stained with DAPI (blue, for nuclear DNA), and anti-tRFP rabbit polyclonal primary antibody plus an Alexa Fluor-488 goat anti-rabbit secondary antibody, prior to epifluorescence imaging. (F) Rgs genes differentially expressed in Tas1r3+ Type II taste receptor cells (responsible for bitter, sweet, and umami taste modalities) versus Type III “presynaptic” taste bud cells (thought responsible for sour and high-salt taste modalities), as identified by RNA-seq transcriptomics (NCBI id SRP094673; bar-graph derived from RNA-seq count data previously published by (Sukumaran et al. 2017)).