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. 2022 Jan 10;11:789462. doi: 10.3389/fcimb.2021.789462

Figure 1.

Figure 1

SARS-CoV-2 Spike inhibits pI:C dependent activation of interferon. (A) IFN-β luciferase reporter assay in HEK 293T cells co-transfected with pI:C (1 μg) and C9 Spike (1 μg). (B) Gene expression levels of IFNb1 determined via Q-PCR in HEK 293T cells co-transfected with pI:C and C9 Spike as in (A). (C) ISRE luciferase reporter assay in HEK 293T cells co-transfected with pI:C and C9 Spike as in (A). (D–F) Gene expression levels of CCL5, CXCL10, and IFIT2 determined via Q-PCR in HEK 293T cells as in (B). (G) NF-ĸB luciferase reporter assay in HEK 293T cells co-transfected with pI:C and C9 Spike as in (A). (H) Gene expression levels of TNF determined via Q-PCR in HEK 293T cells co-transfected with pI:C and C9 Spike as in (B) Data are means ± SEM of one experiment run in triplicate or duplicate out of 2-3 independent experiments. *Indicates p < 0.05 as determined by the Student’s t-test.