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. 2022 Jan 10;11:789462. doi: 10.3389/fcimb.2021.789462

Figure 2.

Figure 2

SARS-CoV-2 Spike promotes higher RNA viral loads. (A) Fluorescent imaging of HEK 293T cells transfected with control vector or C9 Spike (2 µg) (left panels) and subsequently infected with vesicular stomatitis virus (VSV)-GFP (MOI = 0.01) for 16 h. Right panels, bright field image. (B) Relative quantification of GFP intensity from A using ImageJ software. (C) Immunoblot analysis of VSV-GFP in HEK 293T cells transfected with control vector or C9 Spike (2 µg) and infected with VSV-GFP (MOI = 0.01) for 16 h. GAPDH serves as a loading control. (D) Expression levels of Sendai virus (SeV) M gene determined via Q-PCR in HEK 293T cells transfected with C9 Spike (0.25 and 1 µg) and subsequently infected with SeV (MOI = 0.01) for 16 h. *Indicates p < 0.05 as determined by the student’s t-test. Results are representative of two independent experiments. The relative band intensity (*/#) of GFP in (C) was measured using ImageJ software.