FIGURE 2.

Proteomic changes impart a functional deficit in mitochondria in non‐salvageable CLTI. (A) Graphical depiction of novel mitochondrial “stress” test that mimics physiological levels of energy demand. (B) Quantitative analysis of muscle mitochondrial function supported by carbohydrate (pyruvate/malate) and fatty acid (octanoylcarnitine/malate) demonstrate substantial impairment in mitochondrial function in CLTI (n = 10/group). (C) Proteome differences (Log2 fold change) for non‐PAD control and CLTI amputation specimens according to their role in mitochondrial energy transduction. ***P < 0.001, ****P < 0.0001 using two‐tailed t‐test