Figure 1.

Renal ischemia-reperfusion injury (IRI) and cisplatin treatment change gut microbial populations. Under an approved animal protocol, AKI was induced in male, 8- to 10-week-old, C57BL/6 mice by 30 minutes of bilateral IRI or 30 mg/kg cisplatin (CP) injection. The gut microbiota was then studied at baseline (D0) and 72 hours (D3) post-AKI using 16S sequencing. (A) IRI and CP affected relative abundance of bacterial species belonging to the phyla Actinobacteria, Bacteroidetes, Firmicutes, Tenericutes, and Verrucomicrobia. (B) The vertical dot plots represent differential abundance testing between sham and CP mice or sham and IRI mice (x axis, fold change; size, base mean), showing distinct alterations in microbial populations at the family level. The genera identified on the y axis are those that were affected by AKI, using negative binomial testing. There were no significant operational taxonomic units with species-level information. (C and D) Dimensional analysis by Brey non-metric multidimensional scaling (NMDS) and α-diversity analysis using the Shannon index suggests the microbiome differentiates itself over time, depending on treatment. (E) Percentage change in Erysipelotrichaceae incertae sedis and Lactobacillus populations after IRI and CP-induced AKI. Timept, time point. NA, not available.