Renal Mas receptor protein expression in mice after IRI and after LPS administration. Western blot analysis of renal Mas receptor (MAS1) protein expression was determined in mice 1 day after sham or IRI (22.5 minutes) surgery (A) and in mice 15 hours after saline or LPS (10 mg/kg, IV) treatment (B). Equal loading was assessed by immunoblotting of GAPDH, and normalized densitometric analyses for IRI studies (C) and for LPS studies (D) were performed. n = 4 and n = 5 for sham and IRI groups, respectively, in (C), and n = 5 and n = 6 for saline and LPS groups, respectively, in (D); *P<0.05.