Table 2.
Comparison of two common scRNA-seq platforms for droplet microfluidics and split-pool barcoding: 10× Chromium and sci-RNA-seq3
| Parameter | 10× Chromium (Per Lane) | sci-RNA-seq3 (Per Experiment) |
| Throughput | <104 cells | 106 cells |
| Costs per cella | $0.2 | $0.01 |
| Sequencing depth required (per cell) | >30,000 raw reads | >5,000 raw reads |
| Number of detected genes per cell | >2,000 | >500 |
| Labor to generate library | Moderate (1–2 days) | High (1–2 weeks) |
| Data preprocessing methods | User-friendly software (e.g., CellRanger) | Customized pipeline required |
a
Estimated costs for reagents are presented. Illumina sequencing costs are not included.