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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Neurosci Res. 2020 Dec 20;99(4):981–990. doi: 10.1002/jnr.24774

Figure 2. Controlling the Number of Labeled Neurons.

Figure 2.

The number of labeled neurons can be directly controlled by local injection of a Cre-dependent AAV-CIAO-Flp vector, which acts as a gate between anterograde trans-synaptic delivery of AAV1-hSyn1-Cre and a Flp-dependent vector such as AAV-FlpDIO-tdTomato. Because only cells that receive AAV-CIAO-Flp can activate gene expression, reducing the dose of this vector reduces the number of labeled cells without altering the strength of gene expression.