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. 2021 Dec 13;1(8):100132. doi: 10.1016/j.crmeth.2021.100132

Figure 4.

Figure 4

Characterization of spatiotemporally synchronous cells

(A) Correlation between location and timing of individual cells and calcium elevation during synchrony event.

(B) Unsupervised k-mean clustering based on Euclidean distances reveals two categorical clusters of synchronous cells: those that are spatially localized (red) and those that are disperse (blue).

(C) Heatmap of single-cell fluorescence dynamics for clusters 1 and 2.

(D) Comparison of single-cell calcium elevation rate for clusters 1 and 2. Error bars represent standard deviations (unpaired t test, p < 0.001).

(E) MIP spanning the time of high excess synchronicity (90–105 s).

(F and G) Localized region containing cluster 1 cells (F) compared with comparable-sized region (G).

(H–K) Comparison of normalized calcium-dependent fluorescence changes (ΔF/F) and FWHM of clusters 1 and 2. Error bars represent standard deviations (unpaired t test, p = 0.002 for I, p < 0.001 for J)