REAGENT or RESOURCES	SOURCE	IDENTIFIER
Antibodies
Ly6G - clone 1A8	BioLegend	Cat #127616, RRID: AB_1877271
CD11b - clone M1/70	BioLegend	Cat #101226, RRID: AB_830642
NK1.1 -clone PK 136	BioLegend	Cat #108708
Thy1 - clone 53-2.1	BioLegend	Cat #140307, RRID: AB_10643585
Ly6C - clone HK1.4	BioLegend	Cat #128015, RRID AB_1732087
4′,6-diamidino-2-phenylindole (DAPI)	Invitrogen	Cat #D1306
Ter119 - CD90.2	BioLegend	Cat #116208, RRID: AB_313708
Chemicals, peptides, and recombinant proteins
Phosphate-buffered saline 10X (PBS)	Gibco	70011044
Bovine serum albumin (BSA)	MP bio	160069
Red Blood Cell (RBC) Lysing Buffer 10X	Invitrogen	50-112-9743
DMEM - Dulbecco's Modified Eagle Medium (1X)	Gibco	11965-092
DMEM - Dulbecco's Modified Eagle Medium (1X)	Gibco	31053-028
Opti-MEM	Gibco	31985-070
FBS-Fetal Bovine Serum	Corning	35010CV
Penicillin-Streptomycin Solution, 100x	Corning	30-002-CI
Macrophage Colony-Stimulating Factor (Mcsf)	Peprotech	315-02
Collagen type 1	Corning	354236
Lipofectamine 2000 agent	Invitrogen	11668-030
Herring Testis (HT) DNA	Sigma	D6898
Experimental models: Organisms/strains
Mouse: C57CL/6-Tg (Csf1r-cre)1Mnz/J	Jackson Laboratory	029306
Mouse: B6;129S6-Polr2aTn (pb-CAG-GCaMP5g, -tdTomato) Tvrd	Jackson Laboratory	024477
Software and algorithms
FIJI	National Institute of Health, USA	RRID: SCR_002285
Matlab	Mathworks	R2020b
Prism	Graphpad	Version 9.0
Flow cytometer sorter	Sony	MA900
FlowJo	BDBiosciences	N/A
Other
35-mm glass dish	Fluorodish	fd35-100
MC38-H2B-mCherry colon cancer cell lines	Center for Systems biology, Boston, MA	N/A
Fluoview FV1000 confocal microscope	Olympus	FV1000
Vivaview epifluorescence microscope	Olympus	LCV110U
Dorsal skinfold chamber	Custom-built	N/A
Isoflurane	vetone fluriso	26675-46-7
Deposited Data
Image and Signal Processing Code	GitHub / Zenodo	https://doi.org/10.5281/zenodo.5668359