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. Author manuscript; available in PMC: 2022 Sep 30.
Published in final edited form as: Cell. 2021 Sep 23;184(20):5163–5178.e24. doi: 10.1016/j.cell.2021.09.001

Figure 4. Lrp1 binds RVFV glycoprotein Gn.

Figure 4.

A. LRP1 is comprised of four clusters, CLI, CLII, CLIII, and CLIV, and the cytoplasmic and transmembrane domains (left). Mini-domains of CLI, CLII, CLIII, and CLIV were generated as -Fc fusions (top right). Lentiviruses carrying either pLVX-empty vector or pLVX-expressing HA-tagged minidomains mini-LRP1 CLI, CLII, CLIII, and CLIV were also generated (bottom right). B. BV2 WT and Lrp1KO cells were transduced with lentiviruses carrying either pLVX-empty vector (EV) or pLVX-expressing mini-LRP1 CLI, CLII, CLIII, and CLIV prior to infection with RVFV MP12GFP. The bar graph shows the quantification of % infectivity. C. Cell surface expression of mini-LRP1 Clusters in BV2 Lrp1KO cells using flow cytometry for HA. Biolayer interferometry sensograms of RVFV Gn binding to immobilized: D. Fc control, E. Fc-hLrp1 CLII, and F. Fc-hLrp1 CLIV. Neutraliztion of RVFV MP12GFP infection in the presence of exogenous G. Fc control, H. hLrp1 CLII, and I. hLrp1 CLIV.