KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-Lrp1 antibody	Cell Signaling	Cat# 64099, RRID: AB_2799654
Rabbit anti-His antibody	Cell Signaling	Cat# 2365
Mouse anti-tubulin antibody	Sigma Aldrich	Cat# T8328–200UL RRID: AB_1844090
Anti-RVFV clone 4–39-CC	This Paper	N/A
Anti-human IgG Fc (HRP)	Abcam	Cat# ab98624 RRID: AB_10673832
Anti-HA antibody	SantaCruz	sc-805 RRID: AB_631618
Anti-rabbit secondary antibody Alex Fluor-764	Jackson Immuno Research	NC025445
Rabbit anti-RVFV NP	Genescript	N/A
Donkey anti-rabbit Cy3 secondary antibody	Jackson Immuno Research	Cat# 711–165-152 RRID: AB_2307443
Peroxidase-conjugated Donkey Anti-Mouse IgG	Jackson Immuno Research	Cat# 715–035-150 RRID: AB_2340770
Bacterial and virus strains
E. coli BL21 (DE3)	Novagen	Cat# 69450
E. coli Stable3	Thermo Fisher Scientific	Cat# C737303
RVFV ZH501 (reverse genetics generated)	US CDC	N/A
RVFV MP12 GFP	Zhang et al., 2018	N/A
Adenovirus mCherry	Vector Biolabs	Cat# 1767
Adenodvirus mCherry Cre	Vector Biolabs	Cat# 1773
IAV PR8 strain	Williams et al., 2018	N/A
RSV GFP5	Viratree	Cat# R125
VSV-RVFV-eGFP	S. Whelan WashU	N/A
Lentivirus	Addgene vectors	Cat# 8454 RRID: Addgene_8454 Cat# 12260 RRID: Addgene_12260
VSV-eGFP	S. Whelan WashU	N/A
Biological samples
Chemicals, peptides, and recombinant proteins
RVFV Gn (aa 1–316)	this manuscript	N/A
RVFV Gn (aa 1–410)	Genscript	N/A
mRAPD3	this manuscript	N/A
mutant mRAPD3	this manuscript	N/A
human LRP1 CLII- Fc	R & D Systems	Cat# 2368-L2–050
human LRP1 CLIII- Fc	R & D Systems	Cat# 4824-L3–50
human LRP1 CLIV- Fc	R & D Systems	Cat# 5395-L4–050
human IgG1 Fc	R & D Systems	Cat# 110-HG-100
Control VP30 protein	Xu et al., 2017	N/A
Puromycin	Sigma Aldrich	Cat# P8833
Blasticidin	Gibco	Cat# R21001
Lipofectamine 2000	Invitrogen	Cat# 11668019
Surfen (GAG Inhibitor)	Sigma Aldrich	Cat# 362330 100MG
Fetal Bovine Serum	Gibco	Cat# F2442
Fetal Bovine Serum	Gibco	Cat# F2442
HEPES	Sigma Millipore	Cat# 25–060-CI
Sodium pyruvate	Corning	Cat# 25–000-CI
Protein deglycosylation Mix II	New England Biolabs	Cat# P6044S
DMEM Media	Gibco	Cat# 11965084
ammonium acetate, 99.999% purity	Millipore Sigma	372331–100G; CAS 631–61-8
Tris-2(carboxyethyl)phosphine hydrochloride	Millipore Sigma	C4706–10G; CAS 115–96-8
LCMS grade formic acid	CovaChem	11202–10×1; CAS 64–18-6
Triton X-100	Sigma	X100
Permount	Fisher Chemical	Sp15–100
TRIzol Reagent	Ambion	15596018
Seracare TMB 2-C ELISA HRP Substrate	Fisher Scientific	50–674-21
Seracare TMB STOP Solution	Fisher Scientific	50–674-44
Paraformaldehyde	MP Biochemicals	150146
Critical commercial assays
RNeasy Mini Kit	Qiagen	Cat# 74004
Endochrome-K LAL Chromogenic kit	Charles River	Lot# M1153EK1
Power SYBR™ Green PCR Master Mix	ThermoFisher Scientific	Cat# 4367659
SuperScript III Platinum One-Step qRT-PCR Kit	Invitrogen	Cat# 11732088
Deposited data
CRISPR Screen data files	This paper
Experimental models: Cell lines
Human: HEK-293T	ATCC	CRL-3216 RRID: CVCL_0063
Human: SH-SY5Y	ATCC	CRL-2266 RRID: CVCL_0019
Human: HepG2	ATCC	HB-8065 RRID: CVCL_0027
Mouse: BV2	Orvedahl et al., 2019	N/A
African Green Monkey: VeroE6	ATCC	CRL-1586 RRID: CVCL_0574
African Green Monkey: COS1	ATCC	CRL-1650 RRID: CVCL_0223
Bovine: BCE C/D-1b	ATCC	CRL- 2048 RRID: CVCL_2865
Baby Hamster: BHK-21	ATCC	CCL-10 RRID: CVCL_1915
BV2 Lrp1 KO (R1 - R6)	this manuscript	N/A
BV2 Lrp1 PKO R3	this manuscript	N/A
BV2 Lrpap1 KO A7	this manuscript	N/A
BV2 Lrpap1 PKO A3	this manuscript	N/A
BV2 Grp KO (A8, B7)	this manuscript	N/A
BV2 Ext1, Ext2	M. Diamond, Wash U	N/A
Experimental models: Organisms/strains
Mice	The Jackson Laboratory	C57BL/6
Oligonucleotides
GAGTAAACAGGGACACCCGCNGG	Lrp1 5’ gRNA1	N/A
CGGCTCGGGACCCCACTGAGNGG	Lrp1 5’ gRNA2	N/A
GGTTATCAAGGGTAACATGTNGG	Lrp1 3’ gRNA1	N/A
TCTGATTACACCACTTATTGNGG	Lrp1 3’ gRNA2	N/A
CTCCCCGGACTCGCGCTTGGNGG	Lrpap1 (RAP) gRNA	N/A
AAGACCACTCAAATCGAACANGG	Grp94 gRNA	N/A
TGAAAATTCCTGAGACACATGG	RVFV-2912Fwd	N/A
ACTTCCTTGCATCATCTGATG	RVFV-2981Rev	N/A
56FAM/CAATGTAAGGGGCCTGTGTGGACTTGTG/3BHQ_1	RVFV-2950-Probe	N/A
5’- AAGACCAATCCTGTCACCTCTGA-3’	IAV-PR8-M-For	N/A
5’- CAAAGCGTCTACGCTGCAGTCC-3’.	IAV-PR8-M-Rev	N/A
5’-GTCAGCTCATCACCTCAACAA-3’	RVFV-MP12- M seg For	N/A
5’-CACCTGTCATCTGCCTACAAA-3’	RVFV-MP12- M seg Rev	N/A
5’-CTGGTGAAAAGGACCTCTCGAAG-3’	Mouse hprt Fw	N/A
5’-CCAGTTTCACTAATGACACAAACG-3’	Mouse hprt Rev	N/A
pLVX-EF1a	Takara	Cat# 631253
pLVX-mini LRP1 CLI	this manuscript	N/A
pLVX-mini LRP1 CLI	this manuscript	N/A
pLVX-mini LRP1 CLI	this manuscript	N/A
pLVX-mini LRP1 CLI	this manuscript	N/A
pET-mRAP D3	this manuscript	N/A
pET-mutant mRAP D3	this manuscript	N/A
pET-Gn316	this manuscript	N/A
Prism 8.3.0	Graphpad	N/A
BD FACS Diva software™	BD Biosciences	N/A
Octet software	Forte Bio	N/A
FlowJo v10.6.2	FlowJo LLC	N/A
Origin 7	Origin Lab Corporation	N/A
oyager Chromatography Data system v2.0.2.9	Wyatt Technology Corporation	N/A
Astra 7.3	Wyatt Technology Corporation	N/A
Byos® v 3.11	Protein Metrics Inc.	N/A
Freestyle™ v 1.4	Thermo Fisher Scientific Inc.	N/A
DataAnalysis v 4.4	Bruker Corp.	N/A
Leica Application Suite X	Leica	N/A
Other