Figure 2.
Xingxiong injection reduces neuronal injury and apoptosis of rats with MCAO/R and primary cortical neurons with OGD/R. (A) H&E staining in the cortex region in different groups. Scale bar: 200 μm, n = 3; (B) Nissl staining in the cortex region in different groups. Scale bar: 200 μm, n = 3; (C) immunofluorescence for TUNEL-positive neurons in the cortex, n = 3. Scale bar: 50 μm. (D) Caspase-3 activity of primary cortical neurons with OGD/R was measured using a fluorometric assay. (F) Primary cortical neurons were dyed with JC-1 and then detected using a fluorescence microscope. Data are expressed as the mean ± SD and were analysed by ANOVA. ##p < 0.01 vs. control group; **p < 0.01 vs. OGD/R group.