Fig. 3.

Selective immunolabeling of tau aggregates containing tau with four microtubule-binding domain repeats (4R), but not three (3R), in post-mortem human tauopathy brains with antisera targeting JADE1 protein. Immunohistochemical staining with phospho-tau (p-tau) specific antisera (AT8) and JADE1 specific antisera demonstrates neurofibrillary tangle (NFT) formation marked by the presence of JADE1 in specific populations of neurons and glia. a, b Primary age-related tauopathy (PART, n = 3) NFTs contain JADE1 positive staining in the soma and neurites in the entorhinal cortex. c, d Alzheimer disease (AD, n = 3) individual with Aβ and AT8-positive neuritic plaques and NFTs (subiculum) also display JADE1 immunopositivity in dystrophic neurites and NFTs. e, f Chronic traumatic encephalopathy (CTE, n = 3) contains positive p-tau staining around a blood vessel in the depth of a neocortical sulcus that is immunopositive for JADE1. g, h AT8 positive tufted astrocytes, oligodendroglial coiled bodies, and NFTs are positive in the subthalamic nucleus in an individual with progressive supranuclear palsy (PSP, n = 3) which are also in immunopositive for JADE1. i, j Astrocytic plaques in corticobasal degeneration (CBD, n = 3) and extensive thread-like pathology positive for p-tau and JADE1 in the neocortex. k, l In the cornu ammonis 1 (CA1) sector in an individual with argyrophilic grain disease (AGD, n = 3), abundant grains that are immunopositive for p-tau and JADE1 are evident. m, n Pick disease (PiD), a 3R tauopathy, with Pick bodies in the dentate gyrus that are immunopositive for p-tau but negative for JADE1. o Double staining of a PART entorhinal cortex showing the absence of JADE1 (brown) staining in early pre-tangles, but the presence of p-tau (pink, see inset). p Negative control using a peptide competition despite the presence of a tangle (inset). Scale bar, 100 μm