Rats (6 months vs. 24 months old) |
Brain and liver |
Ribosome profiling was conducted as per Ingolia et al. [60,61] using Illumina HiSeq technologies |
Immune and inflammatory response, Lipid oxidation, Stress response, Translation |
Ion channel activity, Neuronal action potential, Lipid biosynthesis, Amine catabolic processes |
[25] |
Yeast |
- |
Replicatively aged yeast cultures were harvested at 15 and 30 hrs and underwent cycloheximide treatment before subsequent polysome and ribosome profiling |
Stress response, Translation repressors |
Ribosome biogenesis, Translational regulators |
[63] |
Mice |
Liver, kidney and skeletal muscle |
Assessed translation efficiency of specific classes of mRNAs using ribosome profiling in 3-month- and 18-month-old mice |
TCA cycle, Oxidative phosphorylation, Fatty acid metabolism, Glycolysis |
mTOR signalling, MAP kinase signalling, Insulin signalling, Translation components |
[64] |
Mice |
Liver and kidney |
Liver and kidney samples were taken across various timepoints with three biological replicates (in all except one condition) undergoing Ribo-seq |
Inflammation and immune response, Lysosome, ECM organisation |
Mitochondrial proteins, Redox homeostasis, Translation components |
[65] |
Human |
Skeletal Muscle |
Skeletal muscle biopsies were performed on three individuals aged between 40–45 and two individuals aged 80+ and the tissues were subjected to ribosome profiling with Illumina HiSeq 2500 short read sequencing |
- |
Mitochondrial proteins, Oxidative phosphorylation |
[66] |
Human |
Heart tissue |
65 left ventricle samples from dilated cardiomyopathy (DCM) and 15 non-DCM controls were used for ribosome profiling. Footprint libraries were sequenced with Illumina HiSeq 2500 |
ECM production, mTOR signalling, Translation components |
Mitochondrial processes |
[46] |