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(A) CRISPR knockout. (B) CRISPR activation. (C) CRISPR interferance, (D) Pooled screens: (1) In vivo, (2) Chemogenetic, (3) Immuno-oncology, and (4) Isogenic screens. (E) Cas9 multiplex platforms: (1) Single Cas9 (e.g. S. pyogenes) system using two copies of the U6 promoter. (2) Single Cas9 system uses two different promoters. (3) A two Cas9, two different promoters system. (F) EnCas12a multiplex platform.
