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. 2021 Nov 23;49(6):2627–2638. doi: 10.1042/BST20210444

Figure 2. Cryo-EM structures for vaccine development.

Figure 2.

(A) Cryo-EM structure of trimeric pre-fusion-stabilized HCMV gB, shown in cartoon representation bound to WAY-174865 (PDB 7KDP), superimposed with tomographic reconstruction [62] (EMD-9328; left panel) and its single-particle cryo-EM map [61] (EMD-22828; middle panel). Three unmodeled regions of density in the map correspond to a Fab fragment from a neutralizing antibody (SM5-1) against gB. Right inset, WAY-174865 binding pocket in gB trimer. (B) Cryo-EM structures of SARS-CoV-2 vaccine antigens BNT162b1 and BNT162b2. Left panel, Cryo-EM structure of dimeric ACE2 (pink) bound to two copies of BNT162b1-encoded SARS-CoV-2 spike protein RBD (blue, PDB 7L7F), superimposed with its cryo-EM map (EMD-23211). Inset shows ACE2-RBD binding interface, with key residues highlighted. Right panel, Cryo-EM structure of trimeric pre-fusion-stabilized SARS-CoV-2 spike protein (PDB 7L7K) encoded by BNT162b2, superimposed with its cryo-EM density (EMD-23215). Subunits are individually colored, with RBD domains separately colored in green.