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. 2022 Jan 6;479(1):57–74. doi: 10.1042/BCJ20210564

Table 1. Kinetic parameters of NgSAT.

Parameter l-serinea Acetyl-CoAa
KM (mM) 1.21 ± 0.16 0.149 ± 0.05
kcat (s−1)b 1444 ± 41 1176 ± 111
kcat/KM (M−1.s−1) 1.19 × 106 ± 0.16 7.89 × 106 ± 2.54
a

Error is SEM of two replicates;

b

kcat calculated by dividing the rate by enzyme concentration using the concentration of the NgSAT hexamer (189.6 kDa).