Table 1. Kinetic parameters of NgSAT.
Parameter | l-serinea | Acetyl-CoAa |
---|---|---|
KM (mM) | 1.21 ± 0.16 | 0.149 ± 0.05 |
kcat (s−1)b | 1444 ± 41 | 1176 ± 111 |
kcat/KM (M−1.s−1) | 1.19 × 106 ± 0.16 | 7.89 × 106 ± 2.54 |
Error is SEM of two replicates;
kcat calculated by dividing the rate by enzyme concentration using the concentration of the NgSAT hexamer (189.6 kDa).