Table 2. Comparison between the two algorithms for 2D classification.

	Selected particles		Time (hh:mm)		Resolution (Å)
	EM	VDAM	EM	VDAM	EM	VDAM
TRPV1	250 182	203 740	03:26	00:50	3.2	3.3
$\gamma$-sec	340 535	350 583	13:49	02:56	3.8	3.8
GDH	476 531	476 559	05:01	00:51	2.5	2.4
CB1	587 385	500 473	12:18	02:46	3.3	3.3
INX6	88 855	89 141	02:04	00:28	4.0	4.0

Good classes were selected manually, and the selected subset was further processed in auto-refine. The number of manually selected particles are shown for each data set and algorithm, as well as the execution time for 2D classification and the final resolution achieved with auto-refine using the subset from the respective subsets.