Table 1.
Cryo-EM structures of mitochondrial translation and ribosome rescue states
| Structure | PDB/EMD code | Description | Ref. |
|---|---|---|---|
| mtPIC1 | PDB 6RW4 | 28S + mtIF3 | [27] |
| mtPIC1 | PDB 6NF8 PDB 6NEQ |
28S + mtIF3 | [45] |
| mtPIC2* (w/o mtIF3) | PDB 6GAZ | 28S + mtIF2 | [17] |
| mtPIC2 | PDB 6RW5 | 28S + mtIF3 + mtIF2 | [27] |
| 55S-IC | PDB 6GAW | 55S + mtIF2 + mRNA + fMet-tRNAfMet (P site) | [17] |
| Decoding complex | PDB 7A5G | 55S + A/T-tRNA/mtEFTu + P/P tRNA/nascent polypeptide + E/E-tRNA | [19] |
| Accommodated complex | PDB 7A5I | 55S + A-tRNA + P-tRNA/nascent polypeptide + E-tRNA | [19] |
| Accommodated complex | PDB 6ZM5 | 55S + A/A-tRNA + P/P-tRNA/nascent polypeptide + OXA1L | [18] |
| Accommodated complex | PDB 6ZSG | 55S + A/A-tRNA + P/P-tRNA + E-tRNA | [15] |
| Rotated-1 complex | EMD-11636 | 55S + A/A-tRNA/nascent polypeptide + P/E-tRNA | [19] |
| Ti Post-translocation (Rotated-2) complex | PDB 6YDW | 55S + A/P-tRNA/nascent polypeptide + P/E-tRNA + mtEFG1*(GDP+Pi) | [48] |
| Rotated-2 complex | PDB 6VLZ | Poorly resolved: 55S/ rotated mtSSU + E-tRNA + mtEFG1*(GMPPCP) | [49] |
| Rotated-2 complex | PDB 6ZSE | 55S + A/P-tRNA + P/E-tRNA | [15] |
| Post-translocation complex | PDB 6VMI | 55S + P-tRNA + E-tRNA + mtEFG1*(GMPPCP) | [49] |
| Post-translocation + Vacant A-site complex |
PDB 6YDP | 55S + P-tRNA/nascent polypeptide + mtEFG1*(GDP+Pi) | [48] |
| Post-translocation complex | PDB 7A5K | 55S + P-tRNA/nascent polypeptide + E-tRNA + mtEFG1*(GMPPCP) | [19] |
| Vacant A-site* (transient state) | PDB 7A5F | 55S + P-tRNA/nascent polypeptide + E-tRNA | [19] |
| Termination complex | PDB 7NQH | 55S + P-tRNA + mtRF1a | [47] |
| Recycling complex-1 | PDB 6ZS9 | 55S + mtRRF | [15] |
| Recycling complex-1 | PDB 7NSI | 55S + P/E-tRNA + mtRRF | [47] |
| Recycling complex-1 | PDB 6NU2 PDB 7L08 |
55S + P/E-tRNA + mtRRF | [45,46] |
| Recycling complex-2 | EMD-23114 | 55S + E-tRNA + mtRRF + mtEFG2*(GMPPCP) | [46] |
| Split mtLSU (canonical recycling) |
PDB 7NSH | 39S + mtRRF + mtEFG2*(GDPNP) | [47] |
| Split mtLSU (canonical recycling) |
PDB 7L20 | 39S + E-tRNA + mtRRF + mtEFG2*(GMPPCP) | [46] |
| Non-stop rescue complex | PDB 7NQL | 55S + P-tRNA + ICT1 | [47] |
| Split mtLSU (alternative recycling) | PDB 7OF4 PDB 7OF6 (distinct PTC conformations) |
39S + GTPBP6*(GTP) | [133] |
| Split mtLSU (mtRQC) |
PDB 7A5H | 39S + P-tRNA/nascent polypeptide + mtRF-R + MTRES1 | [19] |