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. 2022 Jan 25;298(3):101635. doi: 10.1016/j.jbc.2022.101635

Figure 2.

Figure 2

SAMHD1 loss downregulates SARS-CoV-2 and HCoV-OC43 RNA levels in differentiated THP-1 macrophages.A, SAMHD1 expression in PMA-differentiated SAMHD1 WT and KO THP-1 macrophages was confirmed via Western blot using antihuman SAMHD1 antibody. GAPDH was used as a loading control. Separately, using 96-well plates, differentiated SAMHD1 WT and KO THP-1 cells were infected with either (B) SARS CoV-2 or (C) HCoV-OC43 at MOI 0.1 in triplicates, and intracellular RNAs were isolated on days 2 (blue), 3 (red), and 6 (green) postinfection for qRT–PCR analyses. The data are presented as means of triplicates, and the standard deviations from the means are represented as error bars. HCoV-OC43, human coronavirus OC43; MOI, multiplicity of infection; PMA, phorbol 12-myristate 13-acetate; qRT, quantitative RT; SAMHD1, sterile alpha motif and histidine–aspartate domain–containing protein 1; SARS-CoV-2, severe acute respiratory syndrome coronavirus.