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Role of COL5A1 in U251 cell proliferation, migration, and invasion. Efficiency of COL5A1 knockdown in U251 cells analyzed by (a) western blotting and (b) qRT-PCR. (d) Representative photographs of wound healing assay, bar = 100 μm. (e) siRNA-COL5A1 suppressed U251 cells migration. (f, g) Transwell assay of cell migration and invasion. Amplification, ×200. ∗∗∗p < 0.001.
