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HMGA1 was related and bound to RRM2, TK1, and TYMS. (a, b) qRT-PCR and WB were applied to detect the expression of TYMS in EPCs of sepsis patients. (c, d) qRT-PCR and WB were performed to verify TYMS interference efficiency and measure the expression of pyroptosis-related factors ASC, Caspase-1, NLRP3, and IL-18. (e) Correlation Coefficient analysis between HMGA1 and TYMS. (f) Co-IP experiment demonstrated the binding of HMGA1 with TYMS. ∗P < 0.05 vs. Healthy or si-NC.
